FEATURES OF MASS SPECTROMETRIC DETECTION BETA-AMYLOID PEPTIDES (ALZHEIMER'S PEPTIDES)

Mass spectrometry combined with the use of isotope-labeled standards is one favored methods for quantitative measurement beta-amyloid concentrations in biological media. This article devoted to prevention typical systematic errors such measurements arising from neglect possibility transformation into denatured form, which gives mass spectra signals three times more intensity than native form. The degree this denaturation was determined by ability form set up a complex α-2-macroglobulin. lacks point. It shown that can occur case heat treatment an acidic environment or when DMSO used as solvent. Measurement error occurs standard and analyte are different forms. There suggested recommendations overcome analysis these compounds forcing mixture before analysis.